carrier protein crm 197 Search Results


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Synaptic Systems mab anti zinc transporter 3 znt3
a Ventral view of a virtual mouse brain with landmarks for trimming in yellow (left) and horizontal sections from Allen Mouse Brain Atlas (image credit: Allen Institute for Brain Science, available from: https://mouse.brain-map.org/static/brainexplorer ) to illustrate the ventral (level 1, middle panel) and the 600 µm more dorsal region (level 2). b Confocal image of mossy fibers in a Thy1EGFP(M) mouse with zinc-transporter 3 (magenta) staining. A white box marks the imaging window (30 µm × 30 µm) shown enlarged in the next panel. c Two-color d STORM image of synapses stained for presynaptic Bassoon (green) and postsynaptic Homer 1 (magenta). White box indicates magnified view in the next panel. d , e Synaptic contacts in different orientations: upper box—side view, lower box—plane view further magnified in e . f Protein domain layout of Mus musculus Bassoon and Homer1: Zinc-finger (Zn), coiled coil (CC), PxxP, and enabled/VASP homology (EVH); epitopes of the anti-Bassoon (green) and anti-Homer 1 (magenta) antibodies. Histograms of length ( g ) and counts ( i ) of Bassoon clusters in side view at level 1 (blue, n = 181 clusters in nine images from eight animals) and level 2 (magenta, n = 208 clusters in eight images from eight animals). Clusters at level 2 are longer ( p < 0.001) and have more counts ( p < 0.05) than at level 1. Summary box plots (horizontal line median, boxes 25th and 75th, whiskers 10th and 90th percentile) in all eight individual animals for length ( h ) and localization counts ( j ) (* p < 0.05; ** p < 0.01; *** p < 0.001). i Scale bars 100 µm ( b ), 3 µm ( c ), 1 µm ( d ), and 200 nm ( e ).
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a Ventral view of a virtual mouse brain with landmarks for trimming in yellow (left) and horizontal sections from Allen Mouse Brain Atlas (image credit: Allen Institute for Brain Science, available from: https://mouse.brain-map.org/static/brainexplorer ) to illustrate the ventral (level 1, middle panel) and the 600 µm more dorsal region (level 2). b Confocal image of mossy fibers in a Thy1EGFP(M) mouse with zinc-transporter 3 (magenta) staining. A white box marks the imaging window (30 µm × 30 µm) shown enlarged in the next panel. c Two-color d STORM image of synapses stained for presynaptic Bassoon (green) and postsynaptic Homer 1 (magenta). White box indicates magnified view in the next panel. d , e Synaptic contacts in different orientations: upper box—side view, lower box—plane view further magnified in e . f Protein domain layout of Mus musculus Bassoon and Homer1: Zinc-finger (Zn), coiled coil (CC), PxxP, and enabled/VASP homology (EVH); epitopes of the anti-Bassoon (green) and anti-Homer 1 (magenta) antibodies. Histograms of length ( g ) and counts ( i ) of Bassoon clusters in side view at level 1 (blue, n = 181 clusters in nine images from eight animals) and level 2 (magenta, n = 208 clusters in eight images from eight animals). Clusters at level 2 are longer ( p < 0.001) and have more counts ( p < 0.05) than at level 1. Summary box plots (horizontal line median, boxes 25th and 75th, whiskers 10th and 90th percentile) in all eight individual animals for length ( h ) and localization counts ( j ) (* p < 0.05; ** p < 0.01; *** p < 0.001). i Scale bars 100 µm ( b ), 3 µm ( c ), 1 µm ( d ), and 200 nm ( e ).
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Fina BioSolutions crm-197 ecocrm tm -maleimide
a Ventral view of a virtual mouse brain with landmarks for trimming in yellow (left) and horizontal sections from Allen Mouse Brain Atlas (image credit: Allen Institute for Brain Science, available from: https://mouse.brain-map.org/static/brainexplorer ) to illustrate the ventral (level 1, middle panel) and the 600 µm more dorsal region (level 2). b Confocal image of mossy fibers in a Thy1EGFP(M) mouse with zinc-transporter 3 (magenta) staining. A white box marks the imaging window (30 µm × 30 µm) shown enlarged in the next panel. c Two-color d STORM image of synapses stained for presynaptic Bassoon (green) and postsynaptic Homer 1 (magenta). White box indicates magnified view in the next panel. d , e Synaptic contacts in different orientations: upper box—side view, lower box—plane view further magnified in e . f Protein domain layout of Mus musculus Bassoon and Homer1: Zinc-finger (Zn), coiled coil (CC), PxxP, and enabled/VASP homology (EVH); epitopes of the anti-Bassoon (green) and anti-Homer 1 (magenta) antibodies. Histograms of length ( g ) and counts ( i ) of Bassoon clusters in side view at level 1 (blue, n = 181 clusters in nine images from eight animals) and level 2 (magenta, n = 208 clusters in eight images from eight animals). Clusters at level 2 are longer ( p < 0.001) and have more counts ( p < 0.05) than at level 1. Summary box plots (horizontal line median, boxes 25th and 75th, whiskers 10th and 90th percentile) in all eight individual animals for length ( h ) and localization counts ( j ) (* p < 0.05; ** p < 0.01; *** p < 0.001). i Scale bars 100 µm ( b ), 3 µm ( c ), 1 µm ( d ), and 200 nm ( e ).
Crm 197 Ecocrm Tm Maleimide, supplied by Fina BioSolutions, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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R&D Systems polyclonal bmp 2 4 antibody
a Ventral view of a virtual mouse brain with landmarks for trimming in yellow (left) and horizontal sections from Allen Mouse Brain Atlas (image credit: Allen Institute for Brain Science, available from: https://mouse.brain-map.org/static/brainexplorer ) to illustrate the ventral (level 1, middle panel) and the 600 µm more dorsal region (level 2). b Confocal image of mossy fibers in a Thy1EGFP(M) mouse with zinc-transporter 3 (magenta) staining. A white box marks the imaging window (30 µm × 30 µm) shown enlarged in the next panel. c Two-color d STORM image of synapses stained for presynaptic Bassoon (green) and postsynaptic Homer 1 (magenta). White box indicates magnified view in the next panel. d , e Synaptic contacts in different orientations: upper box—side view, lower box—plane view further magnified in e . f Protein domain layout of Mus musculus Bassoon and Homer1: Zinc-finger (Zn), coiled coil (CC), PxxP, and enabled/VASP homology (EVH); epitopes of the anti-Bassoon (green) and anti-Homer 1 (magenta) antibodies. Histograms of length ( g ) and counts ( i ) of Bassoon clusters in side view at level 1 (blue, n = 181 clusters in nine images from eight animals) and level 2 (magenta, n = 208 clusters in eight images from eight animals). Clusters at level 2 are longer ( p < 0.001) and have more counts ( p < 0.05) than at level 1. Summary box plots (horizontal line median, boxes 25th and 75th, whiskers 10th and 90th percentile) in all eight individual animals for length ( h ) and localization counts ( j ) (* p < 0.05; ** p < 0.01; *** p < 0.001). i Scale bars 100 µm ( b ), 3 µm ( c ), 1 µm ( d ), and 200 nm ( e ).
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Nuron Biotech Inc mcc-crm 197 meningitec
a Ventral view of a virtual mouse brain with landmarks for trimming in yellow (left) and horizontal sections from Allen Mouse Brain Atlas (image credit: Allen Institute for Brain Science, available from: https://mouse.brain-map.org/static/brainexplorer ) to illustrate the ventral (level 1, middle panel) and the 600 µm more dorsal region (level 2). b Confocal image of mossy fibers in a Thy1EGFP(M) mouse with zinc-transporter 3 (magenta) staining. A white box marks the imaging window (30 µm × 30 µm) shown enlarged in the next panel. c Two-color d STORM image of synapses stained for presynaptic Bassoon (green) and postsynaptic Homer 1 (magenta). White box indicates magnified view in the next panel. d , e Synaptic contacts in different orientations: upper box—side view, lower box—plane view further magnified in e . f Protein domain layout of Mus musculus Bassoon and Homer1: Zinc-finger (Zn), coiled coil (CC), PxxP, and enabled/VASP homology (EVH); epitopes of the anti-Bassoon (green) and anti-Homer 1 (magenta) antibodies. Histograms of length ( g ) and counts ( i ) of Bassoon clusters in side view at level 1 (blue, n = 181 clusters in nine images from eight animals) and level 2 (magenta, n = 208 clusters in eight images from eight animals). Clusters at level 2 are longer ( p < 0.001) and have more counts ( p < 0.05) than at level 1. Summary box plots (horizontal line median, boxes 25th and 75th, whiskers 10th and 90th percentile) in all eight individual animals for length ( h ) and localization counts ( j ) (* p < 0.05; ** p < 0.01; *** p < 0.001). i Scale bars 100 µm ( b ), 3 µm ( c ), 1 µm ( d ), and 200 nm ( e ).
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ATCC 2 4g2 rat hybridoma
a Ventral view of a virtual mouse brain with landmarks for trimming in yellow (left) and horizontal sections from Allen Mouse Brain Atlas (image credit: Allen Institute for Brain Science, available from: https://mouse.brain-map.org/static/brainexplorer ) to illustrate the ventral (level 1, middle panel) and the 600 µm more dorsal region (level 2). b Confocal image of mossy fibers in a Thy1EGFP(M) mouse with zinc-transporter 3 (magenta) staining. A white box marks the imaging window (30 µm × 30 µm) shown enlarged in the next panel. c Two-color d STORM image of synapses stained for presynaptic Bassoon (green) and postsynaptic Homer 1 (magenta). White box indicates magnified view in the next panel. d , e Synaptic contacts in different orientations: upper box—side view, lower box—plane view further magnified in e . f Protein domain layout of Mus musculus Bassoon and Homer1: Zinc-finger (Zn), coiled coil (CC), PxxP, and enabled/VASP homology (EVH); epitopes of the anti-Bassoon (green) and anti-Homer 1 (magenta) antibodies. Histograms of length ( g ) and counts ( i ) of Bassoon clusters in side view at level 1 (blue, n = 181 clusters in nine images from eight animals) and level 2 (magenta, n = 208 clusters in eight images from eight animals). Clusters at level 2 are longer ( p < 0.001) and have more counts ( p < 0.05) than at level 1. Summary box plots (horizontal line median, boxes 25th and 75th, whiskers 10th and 90th percentile) in all eight individual animals for length ( h ) and localization counts ( j ) (* p < 0.05; ** p < 0.01; *** p < 0.001). i Scale bars 100 µm ( b ), 3 µm ( c ), 1 µm ( d ), and 200 nm ( e ).
2 4g2 Rat Hybridoma, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


a Ventral view of a virtual mouse brain with landmarks for trimming in yellow (left) and horizontal sections from Allen Mouse Brain Atlas (image credit: Allen Institute for Brain Science, available from: https://mouse.brain-map.org/static/brainexplorer ) to illustrate the ventral (level 1, middle panel) and the 600 µm more dorsal region (level 2). b Confocal image of mossy fibers in a Thy1EGFP(M) mouse with zinc-transporter 3 (magenta) staining. A white box marks the imaging window (30 µm × 30 µm) shown enlarged in the next panel. c Two-color d STORM image of synapses stained for presynaptic Bassoon (green) and postsynaptic Homer 1 (magenta). White box indicates magnified view in the next panel. d , e Synaptic contacts in different orientations: upper box—side view, lower box—plane view further magnified in e . f Protein domain layout of Mus musculus Bassoon and Homer1: Zinc-finger (Zn), coiled coil (CC), PxxP, and enabled/VASP homology (EVH); epitopes of the anti-Bassoon (green) and anti-Homer 1 (magenta) antibodies. Histograms of length ( g ) and counts ( i ) of Bassoon clusters in side view at level 1 (blue, n = 181 clusters in nine images from eight animals) and level 2 (magenta, n = 208 clusters in eight images from eight animals). Clusters at level 2 are longer ( p < 0.001) and have more counts ( p < 0.05) than at level 1. Summary box plots (horizontal line median, boxes 25th and 75th, whiskers 10th and 90th percentile) in all eight individual animals for length ( h ) and localization counts ( j ) (* p < 0.05; ** p < 0.01; *** p < 0.001). i Scale bars 100 µm ( b ), 3 µm ( c ), 1 µm ( d ), and 200 nm ( e ).

Journal: Communications Biology

Article Title: Targeted volumetric single-molecule localization microscopy of defined presynaptic structures in brain sections

doi: 10.1038/s42003-021-01939-z

Figure Lengend Snippet: a Ventral view of a virtual mouse brain with landmarks for trimming in yellow (left) and horizontal sections from Allen Mouse Brain Atlas (image credit: Allen Institute for Brain Science, available from: https://mouse.brain-map.org/static/brainexplorer ) to illustrate the ventral (level 1, middle panel) and the 600 µm more dorsal region (level 2). b Confocal image of mossy fibers in a Thy1EGFP(M) mouse with zinc-transporter 3 (magenta) staining. A white box marks the imaging window (30 µm × 30 µm) shown enlarged in the next panel. c Two-color d STORM image of synapses stained for presynaptic Bassoon (green) and postsynaptic Homer 1 (magenta). White box indicates magnified view in the next panel. d , e Synaptic contacts in different orientations: upper box—side view, lower box—plane view further magnified in e . f Protein domain layout of Mus musculus Bassoon and Homer1: Zinc-finger (Zn), coiled coil (CC), PxxP, and enabled/VASP homology (EVH); epitopes of the anti-Bassoon (green) and anti-Homer 1 (magenta) antibodies. Histograms of length ( g ) and counts ( i ) of Bassoon clusters in side view at level 1 (blue, n = 181 clusters in nine images from eight animals) and level 2 (magenta, n = 208 clusters in eight images from eight animals). Clusters at level 2 are longer ( p < 0.001) and have more counts ( p < 0.05) than at level 1. Summary box plots (horizontal line median, boxes 25th and 75th, whiskers 10th and 90th percentile) in all eight individual animals for length ( h ) and localization counts ( j ) (* p < 0.05; ** p < 0.01; *** p < 0.001). i Scale bars 100 µm ( b ), 3 µm ( c ), 1 µm ( d ), and 200 nm ( e ).

Article Snippet: Primary antibodies were used in the following concentrations: mouse monoclonal antibody (mAb) anti-α-Bassoon (Enzo Sap7F407, Enzo Life Sciences, Lörrach, Germany, 1:500), rabbit polyclonal antibody anti-α-Homer 1 (Synaptic Systems, Göttingen, Germany, 106002, 1:500), rabbit polyclonal and mAb anti-Zinc transporter 3 (ZnT3) (Synaptic Systems 197002 and 197011, 1:500), anti GFP nanobodies (GFP-Trap® (uncoupled protein), ChromoTek, Munich, Germany, custom conjugation with Alexa647 , 1:1000).

Techniques: Staining, Imaging